期刊
ACTA BIOMATERIALIA
卷 139, 期 -, 页码 105-117出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2020.12.035
关键词
2D MXene; Stem cells; Regeneration; Neurons; Electrical stimulation, Differentiation
Ti3C2Tx MXene nanosheets have shown the potential to regulate the fate of neural stem cells (NSCs) by promoting their proliferation and neural differentiation. NSCs cultured on Ti(3)C(2)T(x)MXene films exhibit enhanced electrical activity and more mature neurons with longer neurites and increased branching. Electrical stimulation applied through the Ti(3)C(2)T(x)MXene film further enhances NSC proliferation. This study expands the potential applications of MXene materials and provides new strategies for stem cell research.
Preclinical studies involving stem cells require efficient physiochemical regulations on the fate of such cells. Because of their unique planar structure, metallic conductivity, and flexible surface functionalization, MXenes show potential for modulating stem cell fate. Here, the Ti3C2Tx MXenenanosheets are dispersed on tissue culture polystyrene (TCPS). When primary mouse neural stem cells (NSCs) are cultured on laminin-coated Ti3C2TxMXene film, they form stable adhesion, retain their proliferative ability, and show extensive spreading of terminal extensions. With respect to their functional activity, NSCs cultured on Ti(3)C(2)T(x)MXene films form more active and synchronous network activity than those cultured on TCPS substrates. Moreover, Ti(3)C(2)T(x)MXene film significantly promotes the neural differentiation and the neurons have longer neurites and greater numbers of branch points and branch tips. NSC-derived neurons grown on the Ti3C2Tx MXene film preserved normal synapse development. Finally, electrical stimulation coupled with Ti(3)C(2)T(x)MXene film significantly enhances the proliferation of NSCs. These results indicate that Ti3C2Tx MXene is an efficient interface for the proliferation and neural differentiation of NSC and the maturation of NSC-derived neurons, which expands the potential uses of the MXene family of materials and provides new strategies for stem cell studies. Statement of significance The 2DTi(3)C(2)T(x)MXenenanosheets were applied to be an interface for regulating neural stem cells (NSCs). NSCs cultured on Ti(3)C(2)T(x)MXene film possessed higher proliferative ability with higher and more synchronous electrical activities. Moreover, Ti(3)C(2)T(x)MXene film significantly promoted the neural differentiation ratio of NSCs, and the neurons derived from NSCs cultured on Ti(3)C(2)T(x)MXene film had longer neurites and greater numbers of branch points and branch tips.When electrical stimulation was applied to NSCs via the Ti(3)C(2)T(x)MXene film, it significantly enhanced the proliferation of NSCs. This work expands the potential uses of the MXene family of materials and provides new strategies for stem cell studies. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据