期刊
ACS APPLIED MATERIALS & INTERFACES
卷 13, 期 2, 页码 3199-3208出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c20434
关键词
all-in-water microfluidic system; aqueous-droplet-filled hydrogel fiber (ADHF); one-step fabrication; islet organoids; tissue engineering
资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA16020900, XDB32030200, XDB29050301]
- National Science and Technology Major Project [2018ZX09201017-001-001]
- National Nature Science Foundation of China [31971373, 81703470]
- Innovation Program of Science and Research from the DICP, CAS [DICP I201934]
Hydrogel fibers are versatile carriers for biological applications, with flexible mechanical properties and excellent biocompatibility. A new all-in-water microfluidic system enables one-step fabrication of aqueous-droplet-filled hydrogel fibers with unique morphology and tunable configurations, offering high controllability and uniformity.
Hydrogel fibers are promising carriers for biological applications due to their flexible mechanical properties, well-defined spatial distribution, and excellent biocompatibility. In particular, the droplet-filled hydrogel fibers with the controllable dimension and location of droplets display great advantages to enhance the loading capacity of multiple components and biofunctions. In this work, we proposed a new all-in-water microfluidic system that allows for one-step fabrication of aqueous-droplet-filled hydrogel fibers (ADHFs) with unique morphology and tunable configurations. In the system, the aqueous droplets with equidistance are successfully arranged within the alginate calcium fibers, relying on the design of the pump valve cycle and the select of two immiscible liquids with a stable aqueous interface. The architecture of the ADHF can be flexibly controlled by adjusting the three phase flow rates and the valve switch cycle. The produced ADHFs exhibit high controllability, uniformity, biocompatibility, and stability. The established system enabled the formation of functional human islet organoids in situ through encapsulating pancreatic endocrine progenitor cells within microfibers. The generated islet organoids within droplets exhibit high cell viability and islet-specific function of insulin secretion. The proposed approach provides a new way to fabricate multifunctional hydrogel fibers for materials sciences, tissue engineering, and regenerative medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据