期刊
ACS APPLIED MATERIALS & INTERFACES
卷 13, 期 1, 页码 1277-1287出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c19651
关键词
lasting tracking; visual discrimination; carbon quantum dots; peptidoglycan-targeting; Gram-positive bacteria
资金
- National Natural Science Foundation of China [51433008]
- Shaanxi Innovation Capability Support Plan [2020TD-041]
- Shaanxi Key Research AMP
- Development Program Foundation [2020GY-285]
T-SCQDs is a novel method that enables rapid discrimination of peptidoglycan, lasting tracking of bacteria, and nondestructive identification of Gram-positive bacteria within the range of 50-500 μg/mL.
Selective discrimination and lasting tracking of live bacteria are primary steps for microbiology research and treatment of bacterial infection. However, conventional detection methods, such as the gold standard of Gram staining, are being challenged under actual test conditions. Herein, we provided a novel method, namely, three excitation peaks and single-color emission carbon quantum dots (T-SCQDs) for the rapid (5 min) peptidoglycan-targeting discrimination of Gram-positive bacteria and lasting tracking (24 h) through one-step staining. Bacterial viability testing indicates that T-SCQDs can achieve nondestructive identification of Gram-positive bacteria within 50-500 mu g mL(-1). Interestingly, the fluorescence imaging system suggests that T-SCQDs can also selectively distinguish the type of colonies based on fluorescence intensity. Furthermore, T-SCQDs were successfully used to visually distinguish Gram-positive bacteria from the microbial environment of A549 cells by confocal fluorescence microscopy. These properties endow T-SCQDs with excellent functions for the diagnosis of infection and other biological applications.
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