期刊
ISCIENCE
卷 23, 期 11, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2020.101710
关键词
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资金
- Ecole des Neurosciences de Paris (Network for Viral Transfer'')
- Fondation pour la Recherche sur le Cerveau/Rotary Club de France
- Agence Nationale de la Recherche (IHU Institut de Neurosciences Translationelles de Paris) [ANR-10-IAIHU-06]
- OIST Graduate University
- Nakajima Foundation fellowship
Little is known about the real-time cellular dynamics triggered by endogenous catecholamine release despite their importance in brain functions. To address this issue, we expressed channelrhodopsin in locus coeruleus neurons and protein kinase-A activity biosensors in cortical pyramidal neurons and combined two-photon imaging of biosensors with photostimulation of locus coeruleus cortical axons, in acute slices and in vivo. Burst photostimulation of axons for 5-10 s elicited robust, minutes-lasting kinase-A activation in individual neurons, indicating that a single burst firing episode of synchronized locus coeruleus neurons has rapid and lasting effects on cortical network. Responses were mediated by beta 1 adrenoceptors, dampened by co-activation of alpha 2 adrenoceptors, and dramatically increased upon inhibition of noradrenaline reuptake transporter. Dopamine receptors were not involved, showing that kinase-A activation was due to noradrenaline release. Our study shows that noradrenergic transmission can be characterized with high spatiotemporal resolution in brain slices and in vivo using optogenetic tools.
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