4.7 Article

Combined Optogenetic Approaches Reveal Quantitative Dynamics of Endogenous Noradrenergic Transmission in the Brain

期刊

ISCIENCE
卷 23, 期 11, 页码 -

出版社

CELL PRESS
DOI: 10.1016/j.isci.2020.101710

关键词

-

资金

  1. Ecole des Neurosciences de Paris (Network for Viral Transfer'')
  2. Fondation pour la Recherche sur le Cerveau/Rotary Club de France
  3. Agence Nationale de la Recherche (IHU Institut de Neurosciences Translationelles de Paris) [ANR-10-IAIHU-06]
  4. OIST Graduate University
  5. Nakajima Foundation fellowship

向作者/读者索取更多资源

Little is known about the real-time cellular dynamics triggered by endogenous catecholamine release despite their importance in brain functions. To address this issue, we expressed channelrhodopsin in locus coeruleus neurons and protein kinase-A activity biosensors in cortical pyramidal neurons and combined two-photon imaging of biosensors with photostimulation of locus coeruleus cortical axons, in acute slices and in vivo. Burst photostimulation of axons for 5-10 s elicited robust, minutes-lasting kinase-A activation in individual neurons, indicating that a single burst firing episode of synchronized locus coeruleus neurons has rapid and lasting effects on cortical network. Responses were mediated by beta 1 adrenoceptors, dampened by co-activation of alpha 2 adrenoceptors, and dramatically increased upon inhibition of noradrenaline reuptake transporter. Dopamine receptors were not involved, showing that kinase-A activation was due to noradrenaline release. Our study shows that noradrenergic transmission can be characterized with high spatiotemporal resolution in brain slices and in vivo using optogenetic tools.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据