期刊
BMC MOLECULAR BIOLOGY
卷 16, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12867-015-0037-5
关键词
box C/D snoRNP assembly; R2TP complex; Nop17
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/51842-3, 12/51200-7]
- CAPES
- FAPESP postdoctoral fellowships
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/51842-3] Funding Source: FAPESP
Background: Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R2TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R2TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described. Results: To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2. Conclusions: Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R2TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits.
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