期刊
BMC MICROBIOLOGY
卷 15, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s12866-015-0529-y
关键词
Autoinducer-2; Quorum sensing; Biofilm; Pseudomonas aeruginosa
类别
资金
- National Natural Science Foundation of China [81370744]
- Ministry of Education [X3387]
- Scientific Research Foundation of Chongqing Municipal Health Bureau [2013-2-051]
- Scientific Research Foundation of The science and Technology Commission of Chongqing [cstc2015jcyjA10089]
- National Science and Technology [2012BAI04B05]
Background: Pseudomonas aeruginosa is an opportunistic pathogen that is the leading cause of iatrogenic infections in critically ill patients, especially those undergoing mechanical ventilation. In this study, we investigated the effects of the universal signaling molecule autoinducer-2 (AI-2) in biofilm formation of P. aeruginosa PAO1. Results: The addition of 0.1 nM, 1 nM, and 10 nM exogenous AI-2 to P. aeruginosa PAO1 increased biofilm formation, bacterial viability, and the production of virulence factors. However, compared to the 10 nM AI-2 group, higher concentrations of AI-2 (100 nM and 1 mu M) reduced biofilm formation, bacterial viability, and the production of virulence factors. Consistent with the changes in morphology, gene expression analysis revealed that AI-2 up-regulated the expression of quorum sensing-associated genes and genes encoding virulence factors at lower concentrations and down-regulated these genes at higher concentrations. Conclusions: Our study demonstrated that exogenous AI-2 acted in a dose-dependent manner to regulate P. aeruginosa biofilm formation and virulence factors secretion via modulating the expression of quorum sensing-associated genes and may be targeted to treat P. aeruginosa biofilm infections.
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