期刊
CYTOKINE
卷 78, 期 -, 页码 69-78出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2015.11.025
关键词
G-CSF; G-CSF receptor; Colitis; Macrophage
资金
- Natural Sciences & Engineering Research of Canada [RGPIN 312482-2013]
Granulocyte colony-stimulating factor (G-CSF) is a pleiotropic cytokine best known for its role in promoting the generation and function of neutrophils. G-CSF is also found to be involved in macrophage generation and immune regulation; however, its in vivo role in immune homeostasis is largely unknown. Here, we examined the role of G-CSF in dextran sulfate sodium (DSS)-induced acute colitis using G-CSF receptor-deficient (G-CSFR-/-) mice. Mice were administered with 1.5% DSS in drinking water for 5 days, and the severity of colitis was measured for the next 5 days. GCSFR(-/-) mice were more susceptible to DSS-induced colitis than G-CSFR+/+ or G-CSFR-/+ mice. G-CSFR-/- mice harbored less F4/80(+) macrophages, but a similar number of neutrophils, in the intestine. In vitro, bone marrow-derived macrophages prepared in the presence of both G-CSF and macrophage colony-stimulating factor (M-CSF) (G-BMDM) expressed higher levels of regulatory macrophage markers such as programmed death ligand 2 (PDL2), CD71 and CD206, but not in arginase I, transforming growth factor (TGF)-beta, Ym1 (chitinase-like 3) and FIZZ1 (found in inflammatory zone 1), and lower levels of inducible nitric oxide synthase (iNOS), CD80 and CD86 than bone marrow-derived macrophages prepared in the presence of M-CSF alone (BMDM), in response to interleukin (IL)-4/IL-13 and lipopolysaccharide (LPS)/interferon (IFN)-gamma, respectively. Adoptive transfer of G-BMDM, but not BMDM, protected G-CSFR-/- mice from DSS-induced colitis, and suppressed expression of tumor necrosis factor (TNF)-alpha, IL-beta and iNOS in the intestine. These results suggest that G-CSF plays an important role in preventing colitis, likely through populating immune regulatory macrophages in the intestine. (C) 2015 Elsevier Ltd. All rights reserved.
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