期刊
FOODS
卷 9, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/foods9091217
关键词
orange seed; bioactive peptides; antioxidant; ACE-inhibitory; antidiabetic activity; gastrointestinal digestion
资金
- FEDER funds from the Spanish Ministry of Economy, Industry and Competitiveness
- Ramon y Cajal postdoctoral contract
- CSIC [201870E006]
- [AGL2017-89381-R]
In this study, orange seed proteins were hydrolyzed by Alcalase enzyme at different enzyme concentrations 1-3% (v/w) and hydrolysis times (2-5 h), to obtain bioactive peptides showing antioxidant, Angiotensin-converting enzyme (ACE) -inhibitory, and hypoglycemic activities. The highest biological activities (p< 0.05) were achieved by using a hydrolysis time of 5 h and an enzyme concentration of 2%. Orange seed protein hydrolysate (OSPH) was prepared under these conditions, and peptides were isolated and purified by using size-exclusion chromatography and high-performance liquid chromatography, respectively. The fractions that showed the highest biological activities were analyzed by mass spectrometry in tandem, and a total of 63 peptide sequences were found. Moreover, the effect of simulated gastrointestinal digestion on the bioactivity of the fractions was studied, and the novel peptide sequences generated were also identified. Overall, despite there being some differences in the profile of peptide sequences obtained, the main results showed non-significant differences in the analyzed bioactivities after simulated gastrointestinal digestion.
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