期刊
CYTOGENETIC AND GENOME RESEARCH
卷 147, 期 4, 页码 268-273出版社
KARGER
DOI: 10.1159/000444435
关键词
Aegilops tauschii; Amphidiploid; GISH; Intergeneric hybrids; Meiotic chromosome pairing; Secale cereale
资金
- National Natural Science Foundation of China [31271713, 31571649, 31401379]
- Scientific Research Fund of Henan Provincial Education Department [16A210001]
- Henan University [CX0000A40509]
Intergeneric hybrids and amphidiploid hybrids from crosses of Aegilops tauschii and Secale cereale were produced using young embryo rescue. The hybrids showed complete sets of both parental chromosomes. The dihaploid plants showed an average meiotic pairing configuration of 10.84 I + 1.57 II + 0.01 III. Genomic in situ staining revealed 3 types of bivalent associations, i.e. D-D, R-R and D-R at frequencies of 8.6, 8.2 and 83.3%, respectively. Trivalents consisted of D-R-D or R-D-R associations. These results suggested that both intra- and intergenomic chromosome homology were contributed to chromosome pairing. Derived amphidiploids with 2n = 28 paired at metaphase I of meiosis as 4.51 I + 11.70 II + 0.03 III. Chromosome pairing of amphidiploids appeared more or less regular, i.e. bivalent-like with some trivalent configurations. (C) 2016 S. Karger AG, Basel
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