期刊
MICROORGANISMS
卷 8, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms8091339
关键词
arsenic; arsenite oxidation; metabolomics; NMR; mass spectrometry; multi-omics
类别
资金
- National Science Foundation [MCB-1413321, MCB-1714556, MRI:DBI-1532078]
- NIH SIG program [1S10RR13878, 1S10RR026659]
- Murdock Charitable Trust Foundation [2015066:MNL]
- MSU's Vice President for Research and Economic Development's office
- Montana INBRE (NIH) [P20GM103474]
Arsenite (As-III) oxidation is a microbially-catalyzed transformation that directly impacts arsenic toxicity, bioaccumulation, and bioavailability in environmental systems. The genes for As-III oxidation (aio) encode a periplasmic As-III sensor AioX, transmembrane histidine kinase AioS, and cognate regulatory partner AioR, which control expression of the As-III oxidase AioBA. The aio genes are under ultimate control of the phosphate stress response via histidine kinase PhoR. To better understand the cell-wide impacts exerted by these key histidine kinases, we employed H-1 nuclear magnetic resonance (H-1 NMR) and liquid chromatography-coupled mass spectrometry (LC-MS) metabolomics to characterize the metabolic profiles of Delta phoR and Delta aioS mutants of Agrobacterium tumefaciens 5A during As-III oxidation. The data reveals a smaller group of metabolites impacted by the Delta aioS mutation, including hypoxanthine and various maltose derivatives, while a larger impact is observed for the Delta phoR mutation, influencing betaine, glutamate, and different sugars. The metabolomics data were integrated with previously published transcriptomics analyses to detail pathways perturbed during As-III oxidation and those modulated by PhoR and/or AioS. The results highlight considerable disruptions in central carbon metabolism in the Delta phoR mutant. These data provide a detailed map of the metabolic impacts of As-III, PhoR, and/or AioS, and inform current paradigms concerning arsenic-microbe interactions and nutrient cycling in contaminated environments.
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