期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2020.569977
关键词
cerebral ischemic injury; exosomes; circular RNA; autophagy; oxidative stress
资金
- Foundation of Science and Technology Development Fund of Pudong New District Minsheng Scientific Research (Medical and Health) Project [PKJ2017-Y24]
- Discipline Leader in Health Systems of Pudong New District [PWRd2014-09, PwRd2017-10]
- National Natural Science Foundation of China [81201029]
- Key Specialty Construction Project of the Shanghai Municipal Commission of Health and Family Planning [ZK2019A08]
- Municipal Human Resources Development Program for Outstanding Leaders in Medical Disciplines in Shanghai [2017BR051]
- Key Disciplines Group Construction Project of Pudong Health Bureau of Shanghai [PWZxq2017-15]
Background Cerebral ischemic injury is a complicated pathological process. Adipose-derived stromal cells (ADSCs) have been used as a therapeutic strategy, with their therapeutic effects chiefly attributed to paracrine action rather thantrans-differentiation. Studies have shown that circAkap7 was found to be downregulated in a mouse model of transient middle cerebral artery occlusion (tMCAO). Methods To explore whether exosomes derived from circAkap7-modified ADSCs (exo-circAkap7) have therapeutic effects on cerebral ischemic injury, a mouse model of tMCAO, as well as anin vitromodel of oxygen and glucose deprivation-reoxygenation (OGD-R) in primary astrocytes, were used. Results Results showed that treatment with exo-circAkap7 protected against tMCAO in mice, andin vitroexperiments confirmed that co-culture with exo-circAkap7 attenuated OGD-R-induced cellular injury by absorbing miR-155-5p, promoting ATG12-mediated autophagy, and inhibiting NRF2-mediated oxidative stress. Conclusion We demonstrate here that exo-circAkap7 protected against cerebral ischemic injury by promoting autophagy and ameliorating oxidative stress.
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