期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2020.00857
关键词
oocyte maturation; preimplantation mouse embryo; blastocyst cell number; cell-fate; cell lineage decision; cell division
资金
- Czech Science Foundation/GACR grant [18-02891S]
- Grant Agency of the University of South Bohemia Ph.D. student award [GA JU: 015/2017/P]
Formation of the hatching mouse blastocyst marks the end of preimplantation development, whereby previous cell cleavages culminate in the formation of three distinct cell lineages (trophectoderm, primitive endoderm and epiblast). We report that dysregulated expression ofWwc2, a genetic paralog ofKibra/Wwc1(a known activator of Hippo-signaling, a key pathway during preimplantation development), is specifically associated with cell autonomous deficits in embryo cell number and cell division abnormalities. Division phenotypes are also observed during mouse oocyte meiotic maturation, asWwc2dysregulation blocks progression to the stage of meiosis II metaphase (MII) arrest and is associated with spindle defects and failed Aurora-A kinase (AURKA) activation. Oocyte and embryo cell division defects, each occurring in the absence of centrosomes, are fully reversible by expression of recombinant HA-epitope tagged WWC2, restoring activated oocyte AURKA levels. Additionally, clonal embryonic dysregulation implicatesWwc2in maintaining the pluripotent epiblast lineage. Thus,Wwc2is a novel regulator of meiotic and early mitotic cell divisions, and mouse blastocyst cell fate.
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