Engineering disease resistant plants through CRISPR-Cas9 technology

Plants are susceptible to phytopathogens, including bacteria, fungi, and viruses, which cause colossal financial shortfalls (pre- and post-harvest) and threaten global food safety. To combat with these phytopathogens, plant possesses two-layer of defense in the form of PAMP-triggered immunity (PTI), or Effectors-triggered immunity (ETI). The understanding of plant-molecular interactions and revolution of high-throughput molecular techniques have opened the door for innovations in developing pathogen-resistant plants. In this context, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) has transformed genome editing (GE) technology and being harnessed for altering the traits. Here we have summarized the complexities of plant immune system and the use of CRISPR-Cas9 to edit the various components of plant immune system to acquire long-lasting resistance in plants against phytopathogens. This review also sheds the light on the limitations of CRISPR-Cas9 system, regulation of CRISPR-Cas9 edited crops and future prospective of this technology.

Automated summary

Plants are vulnerable to phytopathogens, which can cause significant economic losses and threats to global food safety. Plant immune system has two layers of defense mechanisms, PTI and ETI, while CRISPR-Cas9 technology has revolutionized genome editing for developing pathogen-resistant plants. This review summarizes the complexities of plant immune system, the use of CRISPR-Cas9 for editing plant immune components, and discusses the limitations and future prospects of this technology.