期刊
BMC MICROBIOLOGY
卷 15, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12866-015-0486-5
关键词
Anaplasma phagocytophilum; Ixodes ricinus; TaqMan realtime PCR; Norway; gltA; Prevalence
类别
资金
- Northern Norway Regional Health Authority (Helse Nord RHF) [SFP912-10]
- Scandinavian Society of Antimicrobial Chemotherapy [SLS-100221]
- Division of Medical Services, County Hospital Ryhov, Jonkoping
Background: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Results: Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion: This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.
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