期刊
SCIENCE ADVANCES
卷 6, 期 40, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abc6521
关键词
-
资金
- Hong Kong Research Grants Council [662513, 16103215, 16148816, 16102518, T13-607/12R, T13-706/11-1, T13-605/18W, C6002-17GF, C6001-19E, N_ HKUST603/19]
- Innovation and Technology Commission [ITCPD/17-9]
- Area of Excellence Scheme of the University Grants Committee [AoE/M-604/16, AOE/M-09/12]
- HKUST [RPC10EG33]
Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据