期刊
ACS SENSORS
卷 5, 期 10, 页码 3264-3273出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.0c01734
关键词
ratiometric; near-infrared fluorescent probes; esterase; live and dead cells; mitochondria; zebrafish and mouse imaging
资金
- Natural Science Foundation of China [21771092, 81903561]
- Fundamental Research Funds for the Central Universities [lzujbky-2020-kb13]
- Special Fund Project of Guiding Scientific
- Technological Innovation Development of Gansu Province [2019ZX-04]
- 111 Project [B20027]
- State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China
A series of multifunctional ratiometric near-infrared fluorescent probes (CYOH-3, CYOH-4, CYOH-5, and CYOH-6) for esterase detection are designed by gradually changing the deflection of the plane twist in the molecule. These probes are composed of different ring-structure trigger groups (from threemembered ring to six-membered ring) and the same luminescent group CYOH. These probes show maximum absorption at similar to 585 nm and a fluorescence emission peak at similar to 655 +/- 5 nm. In the presence of esterase, the probes were hydrolyzed to expose the fluorophore CYOH (lambda(abs) = 690 nm, lambda(em) = 710 +/- 5 nm), thus exhibiting ratiometric near-infrared fluorescence. The probe CYOH-6 has lower plane deflection angle and better ratiometric (R = I-710 +/- 5nm/I-657 +/- 4nm) fluorescence properties than probes CYOH-3, CYOH-4, and CYOH-5. CYOH-6 (six-membered ring) has been successfully used to target esterase in mitochondria and distinguish Mi between dead cells (esterase inactivation) and live cells. In addition, CYOH-6 has been well used for monitoring of esterase activity in zebrafish and mice, which proves that these probes have good prospects for clinical biomedical applications.
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