期刊
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
卷 17, 期 3, 页码 263-270出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389201016666150907113435
关键词
Antibacterial activity; hemocyanins; Helix aspersa (HaH); Rapana venosa (RvH); Staphylococcus aureus; Streptococcus epidermidis; Streptococcus pyogenes; Escherichia coli
资金
- Deutsche Forschungsgemeinstchaft, Germany [DFG-STE 1819/5-1/2012]
- Bulgarian Ministry of Education [TK01-496/2009, HTC/01/6]
- European Social Fund and Operational Programme Human Resources Development [DMU 03/26, BG051PO001-3.3.06-0025]
- Bulgarian Ministry of Education, Youth and Science
- Flemish Fund for Scientific Research FWO [VS. 025.12N]
- Bulgarian Academy of Sciences BAS
- Eberhard-Karls-Universitat, Hautklinik, Sektion Dermatologische Onkologie, Tubingen
For the first time the antimicrobial activities of hemocyanins from the molluscs Rapana venosa (RvH) and Helix aspersa (HaH) have been tested. From the hemolymph of the garden snail H. aspersa one structural subunit (beta c-HaH) and eight functional units (FUs, beta c-HaH-a to beta c-HaH-h) were isolated, and their N-terminal sequences and molecular weights, ranging between 45 and 65 kDa, determined. The antimicrobial test of the hemocyanins against different bacteria showed that only two FUs from Rapana, RvH1-b and RvH1-e, exhibit a low inhibition effect against Staphylococcus aureus. In contrast and surprisingly, the structural subunit beta c-HaH of H. aspersa not only shows strong antimicrobial activities against S. aureus and the likewise Gram-positive Streptococcus epidermidis, but also against the Gram-negative bacterium Escherichia coli. We suggest that this subunit therefore has the potential to become a substitute for the commonly used antibiotics against which bacterial resistance has gradually been developed.
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