期刊
FRONTIERS IN GENETICS
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2020.583245
关键词
Bupleurum; saikosaponin biosynthesis; transcriptome analysis; gene expression; P450
资金
- National Natural Science Foundation of China [81603223]
- China Agriculture Research System [CARS-21]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2016-I2M-2-003]
- National Transgenic Major Project of China [2019ZX08010004-005]
- Natural Science Foundation of Science and Technology Department of Sichuan Province [2019YJ0443, 2019YFH0072]
- Young Elite Scientists Sponsorship Program by CACM [2019-QNRC2-C15]
Radix Bupleuri (roots of Bupleurum spp.) is an important medicinal herb. Triterpenoid saponins of saikosaponins generally constitute the main class of secondary metabolites of plants in the Bupleurum genus. However, the molecular regulatory mechanism underlying their biosynthesis remains elusive. In this study, we observed significantly different saikosaponin biosynthesis between Bupleurum chinense and Bupleurum scorzonerifolium at the seedling stage. The sequential and expression characterization of 232 genes in the triterpenoid saponin biosynthetic pathway, which includes the mevalonate (MVA) pathway and methylerythritol phosphate (MEP) pathway, between B. chinense and B. scorzonerifolium was also investigated. Sixty of these genes may be involved in saikosaponin biosynthesis. Manipulation of these genes, especially those of the beta-AS, P450, and UGT families, may improve saikosaponin production.
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