期刊
REDOX BIOLOGY
卷 37, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.redox.2020.101740
关键词
Heart failure; Manganese superoxide dismutase; Superoxide radicals
资金
- Louisiana State University Health Sciences-Shreveport Intramural Grant [110101074A]
- National Institutes of Health [HL141998, HL141998-01S1, AA025744, AA026708, AA025744-02S1, AA023610, HL122354, HL145753, P20GM121307]
Electrophilic aldehyde (4-hydroxynonenal; 4-HNE), formed after lipid peroxidation, is a mediator of mitochondrial dysfunction and implicated in both the pathogenesis and the progression of cardiovascular disease. Manganese superoxide dismutase (MnSOD), a nuclear-encoded antioxidant enzyme, catalyzes the dismutation of superoxide radicals (O-2(center dot-)) in mitochondria. To study the role of MnSOD in the myocardium, we generated a cardiomyocyte-specific SOD2 (SOD2 Delta) deficient mouse strain. Unlike global SOD2 knockout mice, SOD2 Delta mice reached adolescence; however, they die at similar to 4 months of age due to heart failure. Ultrastructural analysis of SOD2 Delta hearts revealed altered mitochondrial architecture, with prominent disruption of the cristae and vacuole formation. Noninvasive echocardiographic measurements in SOD2 Delta mice showed dilated cardiomyopathic features such as decreased ejection fraction and fractional shortening along with increased left ventricular internal diameter. An increased incidence of ventricular tachycardia was observed during electrophysiological studies of the heart in SOD2 Delta mice. Oxidative phosphorylation (OXPHOS) measurement using a Seahorse XF analyzer in SOD2 Delta neonatal cardiomyocytes and adult cardiac mitochondria displayed reduced O-2 consumption, particularly during basal conditions and after the addition of FCCP (H+ ionophore/uncoupler), compared to that in SOD2fi hearts. Measurement of extracellular acidification (ECAR) to examine glycolysis in these cells showed a pattern precisely opposite that of the oxygen consumption rate (OCR) among SOD2 Delta mice compared to their SOD2(fl) littermates. Analysis of the activity of the electron transport chain complex identified a reduction in Complex I and Complex V activity in SOD2 Delta compared to SOD2f1 mice. We demonstrated that a deficiency of SOD2 increases reactive oxygen species (ROS), leading to subsequent overproduction of 4-HNE inside mitochondria. Mechanistically, proteins in the mitochondrial respiratory chain complex and TCA cycle (NDUFS2, SDHA, ATP5B, and DLD) were the target of 4-HNE adduction in SOD2 Delta hearts. Our findings suggest that the 5OD2 mediated 4-HNE signaling nexus may play an important role in cardiomyopathy.
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