期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 41, 期 -, 页码 90-97出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2016.06.004
关键词
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资金
- University of Southampton
- Engineering and Physical Sciences Research Council (EPSRC)
- Biotechnology and Biological Sciences Research Council
- EPSRC
Hydrogenases are a potential source of environmentally benign bioenergy, using complex cofactors to catalyze the reversible reduction of protons to form hydrogen. The most active subclass, the [FeFe]-hydrogenases, is dependent on a metallocofactor, the H cluster, that consists of a two iron subcluster ([2Fe](H)) bridging to a classical cubane cluster ([4Fe-4S](H)). The ligands coordinating to the diiron subcluster include an azadithiolate, three carbon monoxides, and two cyanides. To assemble this complex cofactor, three maturase enzymes, HydG, HydE and HydF are required. The biosynthesis of the diatomic ligands proceeds by an unusual fragmentation mechanism, and structural studies in combination with spectroscopic analysis have started to provide insights into the HydG mediated assembly of a [2Fe](H) subcluster precursor.
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