期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 41, 期 -, 页码 225-232出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2016.09.006
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资金
- Howard Hughes Medical Institute
Macromolecular complexes consisting of proteins, lipids, and/or nucleic acids are ubiquitous in biological processes. Their composition, stoichiometry, order of assembly, and conformations can be heterogeneous or can change dynamically, making single-molecule studies best suited to measure these properties accurately. Recent single-molecule pull-down and other related approaches have combined the principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native macromolecular complexes. In this review, we present the advances in single-molecule pull-down methods and biological systems that have been investigated in such semi vivo manner.
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