Applications of atomic force microscopy in immunology

Cellular mechanics, a major regulating factor of cellular architecture and biological functions, responds to intrinsic stresses and extrinsic forces exerted by other cells and the extracellular matrix in the microenvironment. Cellular mechanics also acts as a fundamental mediator in complicated immune responses, such as cell migration, immune cell activation, and pathogen clearance. The principle of atomic force microscopy (AFM) and its three running modes are introduced for the mechanical characterization of living cells. The peak force tapping mode provides the most delicate and desirable virtues to collect high-resolution images of morphology and force curves. For a concrete description of AFM capabilities, three AFM applications are discussed. These applications include the dynamic progress of a neutrophil-extracellular-trap release by neutrophils, the immunological functions of macrophages, and the membrane pore formation mediated by perforin, streptolysin O, gasdermin D, or membrane attack complex.

Automated summary

Cellular mechanics is a major regulating factor of cellular architecture and biological functions, responding to intrinsic and extrinsic forces in the microenvironment. It plays a fundamental role in complicated immune responses and can be characterized using atomic force microscopy (AFM). AFM applications include studying neutrophil-extracellular-trap release, immunological functions of macrophages, and membrane pore formation.