期刊
FRONTIERS IN IMMUNOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2020.535784
关键词
CD21(low) B cells; antigen-presenting cells; autoimmunity; co-stimulation; T cell
类别
资金
- Deutsche Forschungsgemeinschaft [TRR130, P07 toKWand P12]
- German Federal Ministry of Education and Research [BMBF 01E01303]
Human CD21(low) B cells are expanded in autoimmune (AI) diseases and display a unique phenotype with high expression of co-stimulatory molecules, compatible with a potential role as antigen-presenting cells (APCs). Thus, we addressed the co-stimulatory capacity of naive-like, IgM-memory, switched memory and CD27(neg)IgD(neg) memory CD21(low) B cells in allogenic co-cultures with CD4 T cells. CD21(low) B cells of patients with AI disorders expressed high levels of not only CD86, CD80, and HLA-DR (memory B cells) but also PD-L1 ex vivo and efficiently co-stimulated CD4 T cells of healthy donors (HD), as measured by upregulation of CD25, CD69, inducible co-stimulator (ICOS), and programmed cell death protein 1 (PD-1) and induction of cytokines. While the co-stimulatory capacity of the different CD21(low) B-cell populations was over all comparable to CD21(pos) counterparts of patients and HD, especially switched memory CD21(low) B cells lacked the increased capacity of CD21(pos) switched memory B-cells to induce high expression of ICOS, IL-2, IL-10, and IFN-gamma. Acknowledging the limitation of the in vitro setting, CD21(low) B cells do not seem to preferentially support a specific T-h effector response. In summary, our data implies that CD21(low) B cells of patients with AI diseases can become competent APCs and may, when enriched for autoreactive B-cell receptors (BCR), potentially contribute to AI reactions as cognate interaction partners of autoreactive T cells at sites of inflammation.
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