期刊
FRONTIERS IN IMMUNOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2020.02003
关键词
pancreatic cancer; pancreatic cyst neoplasm; IPMN; oral commensals; humoral response
类别
资金
- Swedish Cancer Society [CAN2016/731, 19 0495 Pj 01 H, CAN 2017/409, CAN2017/419]
- Karolinska Institutet funds
- SOF Strategic Odontological Research
- Radiumhemmets forskningsfonder
- ALF [SLL20150113]
Objectives Intraductal papillary mucinous neoplasms (IPMNs) are cystic precursor lesions to pancreatic cancer. The presence of oral microbes in pancreatic tissue or cyst fluid has been associated with high-grade dysplasia (HGD) and cancer. The present study aims at investigating if humoral immunity to pancreas-associated oral microbes reflects IPMN severity. Design Paired plasma (n= 109) and saliva (n= 65) samples were obtained from IPMN pancreatic cystic tumor cases and controls, for anti-bacterial antibody analysis and DNA quantification by enzyme-linked immunosorbent assay (ELISA) and qPCR, respectively. Tumor severity was graded by histopathology, laboratory, and clinical data. Circulating plasma and salivary antibody reactivity to a pancreas-associated oral microbe panel were measured by ELISA and correlated to tumor severity. Results The patient group with high-risk cystic tumors (HGD and/or associated invasive cancer) shows ample circulating IgG reactivity toFusobacterium nucleatum(F. nucleatum) but not toGranulicatella adiacens(G. adiacens), which is independent of the salivary bacteria DNA levels. This group also shows higher salivary IgA reactivity toF. nucleatum, Fap2 ofF. nucleatum, andStreptococcus gordonii(S. gordonii) compared to low-risk IPMN and controls. The salivary antibody reactivity toF. nucleatumand Fap2 are found to be highly correlated, and cross-competition assays further confirm that these antibodies appear cross-reactive. Conclusion Our findings indicate that humoral reactivity against pancreas-associated oral microbes may reflect IPMN severity. These findings are beneficial for biomarker development.
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