Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of Lactarius deliciosus

Fungi play an important role in dying wastewater treatment. In this work, the mycelia ofLactarius deliciosusexhibited an excellent capacity in decolorizing coomassie brilliant blue (CBB). The results demonstrated that the mycelia could treat CBB with high concentrations over a broad range of pH and temperature. The decolorization rate of 99.19% and the removal rate of 16.31 mg.L-1.h were realized. The mycelia could be recycled from decolorizing process for 19 times, indicating a good re-usability. It verified that the lignin peroxidase (121.65 U.L-1) and manganese peroxidase (36.77 U.L-1) were involved in the degradation and decolorization process of CBB. Toxicity assessments indicated the seed germination rate was up to 82.22% while inhibition toEscherichia colidecreased dramatically and no significant effect onCaenorhabditis elegansgrowth was found. The removal of CBB was a synergistic process accomplished by adsorption and biodegradation. The mycelia could be used for eco-friendly CBB treatment.

Automated summary

The mycelia of Lactarius deliciosus showed excellent capability in decolorizing CBB, with high removal rates and good re-usability. Enzymes involved in the degradation process were verified, and toxicity assessments indicated minimal impact on seed germination and bacterial growth. The removal of CBB was achieved through a synergistic process of adsorption and biodegradation.