期刊
CURRENT OPINION IN PLANT BIOLOGY
卷 30, 期 -, 页码 70-77出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.pbi.2016.01.007
关键词
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资金
- Department of Energy (DOE), Office of Science, Genomic Science Program [DE-SC0008834]
- US DOE [DE-AC05-00OR22725]
Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. This article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, and identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.
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