期刊
3 BIOTECH
卷 10, 期 9, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-020-02397-w
关键词
Mushroom; Trametes versicolor; HPLC; FTIR; Anti-inflammatory
In the present study, modified solvent evaporation assisted methanolicTrametes versicolorextract was evaluated for its antimicrobial and anti-inflammatory efficacy. Mushroom extract showed significantly (p < 0.05) higher total phenolic content (48.71 mg/g) followed by total flavonoid content (13.13 mg/g), ascorbic acid content (11.03 mg/g), beta-carotene content (8.34), and lycopene content (6.85). Fourier transform infrared spectroscopy revealed the functional groups of the observed bioactive compounds, while HPLC chromatogram showed significantly (p < 0.05) higher gallic acid content (45.72 mg/g) as compared to rutin content (12.50 mg/g). Statistically, mushroom extract and artificial antioxidant (BHA) showed a non-significant (p < 0.05) difference in terms of percentage inhibition during DPPH and N(2)O(2)scavenging assay. During the time-kill kinetics, the mushroom extract significantly inhibited the growth ofS. aureusin comparison with the growth ofP.aeruginosa,K. pneumonia,andE.coli. Mushroom extract showed effective anti-inflammatory activity during membrane stabilization (33.71-73.24%) and protein denaturation (23.11-74.56%) assays.
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