期刊
NANOMATERIALS
卷 10, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/nano10102044
关键词
nanoporous alumina membrane; fluorescence resonance energy transfer; Au@ZIF-8 nanoparticles; DNA oxidation damage; 8-OHdG detection
类别
资金
- National Natural Science Foundation of China (NSFC) [81601570]
- Zhejiang Provincial Natural Science Foundation of China [LY20D060008]
- Science and Technology Department of Zhejiang Province [2019C03066]
A sensitive fluorescence resonance energy transfer (FRET) biosensor is proposed to detect 8-hydroxy-2 '-deoxyguanosine (8-OHdG), which is a typical DNA oxidation damage product excreted in human urine. The FRET biosensor was based on carbon dots (CDs)-modified nanoporous alumina membrane with CDs as fluorescence donors. Gold nanoparticles were encapsulated in zeolitic imidazolate framework-8 to form Au@ZIF-8 nanoparticles as signal quenchers. CDs and Au@ZIF-8 nanoparticles were biofunctionalized by 8-OHdG antibody. The capture of 8-OHdG on the membrane substrates can bring Au@ZIF-8 nanoparticles closely to CDs. With 350 nm excitation, the fluorescence of CDs was quenched by Au@ZIF-8 nanoparticles and FRET effect occurred. The quenching efficiency was analyzed. The limit of detection (LOD) was 0.31 nM. Interference experiments of the FRET biosensor showed good specificity for 8-OHdG detection. The biosensor could detect urinary 8-OHdG sensitively and selectively with simple sample pretreatment processes. It shows applicability for detecting biomarkers of DNA damage in urine or other biological fluids.
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