4.4 Article

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

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出版社

JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/61640

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资金

  1. INSERM, Universite Cote d'Azur
  2. French National Research Agency (ANR) through the Investments for the Future Labex SIGNALIFE [ANR-11-LABX-0028-01]
  3. program UCA JEDI via Academy 2 Systemes Complexes [ANR-15-IDEX-01]
  4. program UCA JEDI via Academy 4 Complexite et diversite du vivant [ANR-15-IDEX-01]
  5. Fondation pour la Recherche Medical [Equipe FRM DEQ20180839587]
  6. Young Investigator Program [ANR18-CE14-0035-01-GILLERON]
  7. Conseil Departemental des Alpes-Maritimes
  8. Region PACA
  9. IBISA Microscopy and Imaging Platform Cote d'Azur (MICA)

向作者/读者索取更多资源

Obesity is a major worldwide public health issue that increases the risk to develop cardiovascular diseases, type-2 diabetes, and liver diseases. Obesity is characterized by an increase in adipose tissue (AT) mass due to adipocyte hyperplasia and/or hypertrophia, leading to profound remodeling of its three-dimensional structure. Indeed, the maximal capacity of AT to expand during obesity is pivotal to the development of obesity-associated pathologies. This AT expansion is an important homeostatic mechanism to enable adaptation to an excess of energy intake and to avoid deleterious lipid spillover to other metabolic organs, such as muscle and liver. Therefore, understanding the structural remodeling that leads to the failure of AT expansion is a fundamental question with high clinical applicability. In this article, we describe a simple and fast clearing method that is routinely used in our laboratory to explore the morphology of mouse and human white adipose tissue by fluorescent imaging. This optimized AT clearing method is easily performed in any standard laboratory equipped with a chemical hood, a temperature-controlled orbital shaker and a fluorescent microscope. Moreover, the chemical compounds used are readily available. Importantly, this method allows one to resolve the 3D AT structure by staining various markers to specifically visualize the adipocytes, the neuronal and vascular networks, and the innate and adaptive immune cells distribution.

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