期刊
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
卷 44, 期 5, 页码 1085-1090出版社
SPRINGER
DOI: 10.1007/s40618-020-01436-w
关键词
Thyroid; Thyrocytes; SARS-COV-2; COVID-19; ACE-2
资金
- Universita degli Studi di Pavia within the CRUI-CARE Agreement
The study demonstrated the expression of ACE-2 receptor mRNA in thyroid follicular cells, making them a potential target for SARS-COV-2 entry. Future clinical studies in COVID-19 patients will be needed to further understand the impact of SARS-CoV-2 infection on the thyroid.
Purpose SARS-COV-2 is a pathogenic agent belonging to the coronavirus family, responsible for the current global world pandemic. Angiotensin-converting enzyme 2 (ACE-2) is the receptor for cellular entry of SARS-CoV-2. ACE-2 is a type I transmembrane metallo-carboxypeptidase involved in the Renin-Angiotensin pathway. By analyzing two independent databases, ACE-2 was identified in several human tissues including the thyroid. Although some cases of COVID-19-related subacute thyroiditis were recently described, direct proof for the expression of the ACE-2 mRNA in thyroid cells is still lacking. Aim of the present study was to investigate by RT-PCR whether the mRNA encoding for ACE-2 is present in human thyroid cells. Methods RT-PCR was performed on in vitro ex vivo study on thyroid tissue samples (15 patients undergoing thyroidectomy for benign thyroid nodules) and primary thyroid cell cultures. Results The ACE-2 mRNA was detected in all surgical thyroid tissue samples (n = 15). Compared with two reporter genes (GAPDH: 0.052 +/- 0.0026 Cycles(-1); beta-actin: 0.044 +/- 0.0025 Cycles(-1); ACE-2: 0.035 +/- 0.0024 Cycles(-1)), the mean level of transcript expression for ACE-2 mRNA was abundant. The expression of ACE-2 mRNA in follicular cells was confirmed by analyzing primary cultures of thyroid cells, which expressed the ACE-2 mRNA at levels similar to tissues. Conclusions The results of the present study demonstrate that the mRNA encoding for the ACE-2 receptor is expressed in thyroid follicular cells, making them a potential target for SARS-COV-2 entry. Future clinical studies in patients with COVID-19 will be required for increase our understanding of the thyroid repercussions of SARS-CoV-2 infection.
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