期刊
GENES
卷 11, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/genes11091088
关键词
antibiotic resistance genes; dietary habits; human saliva; human salivary resistome; oral cavity
资金
- Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR)
Food consumption allows the entrance of bacteria and their antibiotic resistance (AR) genes into the human oral cavity. To date, very few studies have examined the influence of diet on the composition of the salivary microbiota, and even fewer investigations have specifically aimed to assess the impact of different long-term diets on the salivary resistome. In this study, the saliva of 144 healthy omnivores, ovo-lacto-vegetarians, and vegans were screened by nested PCR for the occurrence of 12 genes conferring resistance to tetracyclines, macrolide-lincosamide-streptogramin B, vancomycin, and beta-lactams. Thetet(W),tet(M), anderm(B) genes occurred with the highest frequencies. Overall, no effect of diet on AR gene distribution was seen. Some differences emerged at the recruiting site level, such as the higher frequency oferm(C) in the saliva of the ovo-lacto-vegetarians and omnivores from Bologna and Turin, respectively, and the higher occurrence oftet(K) in the saliva of the omnivores from Bologna. A correlation of the intake of milk and cheese with the abundance oftet(K) anderm(C) genes was seen. Finally, when the occurrence of the 12 AR genes was evaluated along with geographical location, age, and sex as sources of variability, high similarity among the 144 volunteers was seen.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据