期刊
CATALYSTS
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/catal10090947
关键词
L-arginine; putrescine; Escherichia coli; whole-cell catalysis; co-expression
资金
- National Key Scientific Instrument and Equipment Development Project of China [2013YQ17052504]
- Program for Changjiang Scholars and Innovative Research Teams in the University of Ministry of Education of China [IRT_15R55]
- Natural Science Foundation of Shanxi province [2019JQ-725]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX19_1841, KYCX19_1842]
Putrescine, a biogenic amine, is a highly valued compound in medicine, industry, and agriculture. In this study, we report a whole-cell biocatalytic method inEscherichia colifor the production of putrescine, using L-arginine as the substrate. L-arginine decarboxylase and agmatine ureohydrolase were co-expressed to produce putrescine from L-arginine. Ten plasmids with different copy numbers and ordering of genes were constructed to balance the expression of the two enzymes, and the best strain was pACYCDuet-speB-speA. The optimal concentration of L-arginine was determined to be 20 mM for this strain. The optimum pH of the biotransformation was 9.5, and the optimum temperature was 45 degrees C; under these conditions, the yield of putrescine was 98%. This whole-cell biocatalytic method appeared to have great potential for the production of putrescine.
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