期刊
MICROMACHINES
卷 11, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/mi11090882
关键词
cell elasticity; endothelial cells; optical micromanipulation; holographic optical tweezers; two-photon polymerization; image processing
类别
资金
- NVIDIA Corporation
- Ministry of Innovation and Technology, Hungary [UNKP-20-4-SZTE-138]
- [GINOP-2.3.2-15-2016-00001]
- [GINOP-2.3.3-15-2016-00040]
A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young's modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据