期刊
FRONTIERS IN MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.574882
关键词
Penicillium digitatum; peptide; C12O3TR; RNA-Seq; cell wall
类别
资金
- National Key R&D Program of China [2018YFD0401301]
- Project of Chongqing Science and Technology Bureau, China [cstc2019jscxdxwtBX0027]
In this study, the transcriptional profiling ofPenicillium digitatumafter C12O3TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes inP. digitatumwere differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C12O3TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of beta-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C12O3TR could damage the cell wall integrity. In conclusion, these results suggested that C12O3TR could inhibit the growth ofP. digitatumthrough various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity.
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