期刊
OPEN BIOLOGY
卷 10, 期 9, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsob.200209
关键词
coronavirus; envelope protein; membrane insertion; SARS-CoV-2; topology
资金
- Generalitat Valenciana [PROMETEU/2019/065]
- ISCIII [COV20/01265]
Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt(lum)/Ct(cyt)). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.
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