期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 41, 期 -, 页码 114-121出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2016.06.007
关键词
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资金
- Dept. of Isotope Biogeochemistry, Centre for Chemical Microscopy (ProVIS), Helmholtz Centre for Environmental Research (UFZ)
- U.S. Department of Energy Genomic Science Program [SCW1039]
- U.S. Department of Energy [DE-AC52-07NA27344]
- National Science Foundation (USA) via NSF [OCE-1136727]
The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe identification via fluorescence in situ hybridization (FISH) is often used to link identity to function at the cellular level in microbial communities. Many opportunities remain for nanoSIP to identify metabolic interactions and nutrient fluxes within syntrophic associations and obligate symbioses where exchanges can be extremely rapid. However, additional data, such as genomic potential, gene expression or other imaging modalities are often critical to deciphering the mechanisms underlying specific interactions, and researchers must keep sample preparation artefacts in mind. Here we focus on recent applications of nanoSlP, particularly where used to track exchanges of isotopically labelled molecules between organisms. We highlight metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont-host nutrient sharing.
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