期刊
SCIENTIFIC REPORTS
卷 10, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41598-020-73446-w
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资金
- DARPA-NESD [N66001-17-C-4012]
- NSF Instrument Development for Biological Research Award [1556164, 1556213, 1257555]
- FAPESP [2017/26279-2, 2014/18541-0]
- DOE EvoNet Grant [DE-SC0014377]
- U.S. Department of Energy (DOE) [DE-SC0014377] Funding Source: U.S. Department of Energy (DOE)
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1556213] Funding Source: National Science Foundation
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1556164] Funding Source: National Science Foundation
Pyrosomes are tunicates in the phylum Chordata, which also contains vertebrates. Their gigantic blooms play important ecological and biogeochemical roles in oceans. Pyrosoma, meaning fire-body, derives from their brilliant bioluminescence. The biochemistry of this light production is unknown, but has been hypothesized to be bacterial in origin. We found that mixing coelenterazine-a eukaryote-specific luciferin-with Pyrosoma atlanticum homogenate produced light. To identify the bioluminescent machinery, we sequenced P. atlanticum transcriptomes and found a sequence match to a cnidarian luciferase (RLuc). We expressed this novel luciferase (PyroLuc) and, combined with coelenterazine, it produced light. A similar gene was recently predicted from a bioluminescent brittle star, indicating that RLuc-like luciferases may have evolved convergently from homologous dehalogenases across phyla (Cnidaria, Echinodermata, and Chordata). This report indicates that a widespread gene may be able to functionally converge, resulting in bioluminescence across animal phyla, and describes and characterizes the first putative chordate luciferase.
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