期刊
MATERIALS
卷 13, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/ma13194336
关键词
propionic acid; autophagy; mitophagy; correlative light and electron microscopy (CLEM)
类别
资金
- National Research Foundation of Korea (NRF) - government of Korea (MSIP) [2019R1A2C1010634]
- Organelle Network Research Center [NRF-2017R1A5A1015366]
- Korea Brain Research Institute - Ministry of Science and ICT [20-BR-02-09]
- National Research Foundation of Korea [2019R1A2C1010634] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Propionic acid is a metabolite of the microbiome and can be transported to the brain. Previous data show that propionic acid changes mitochondrial biogenesis in SH-SY5Y cells and induces abnormal autophagy in primary hippocampal neurons. Maintaining mitochondrial function is key to homeostasis in neuronal cells, and mitophagy is the selective autophagy involved in regulating mitochondrial quality. Monitoring mitophagy though light microscopy or conventional transmission electron microscopy separately is insufficient because phases of mitophagy, including autophagosome and autolysosome in nano-resolution, are critical for studies of function. Therefore, we used correlative light and electron microscopy to investigate mitochondrial quality in SH-SY5Y cells after propionic acid treatment to use the advantages of both techniques. We showed, with this approach, that propionic acid induces mitophagy associated with mitochondrial quality.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据