期刊
CONSERVATION GENETICS RESOURCES
卷 12, 期 4, 页码 691-700出版社
SPRINGER
DOI: 10.1007/s12686-020-01165-5
关键词
Amphibians; Application; Conservation genetics; Conservation practice; eDNA metabarcoding; Gene flow
资金
- Lib4RI - Library for the Research Institutes within the ETH Domain: Eawag, Empa, PSI WSL
- InnoSuisse [19204.1 PFLS-LS]
- Swiss Federal Office for the Environment BAFU
- Swiss canton of Argovia
- Swiss canton of Appenzell Ausserrhoden
- Swiss canton of Berne
- Swiss canton of Geneva
- Swiss canton of Lucerne
- Swiss canton of Ob- and Nidwalden
- Swiss canton of Schaffhausen
- Swiss canton of Thurgau
- Swiss canton of Uri
- Swiss canton of Zug
- Swiss canton of Zurich
- Austrian state Salzburg
We present a conservation genetics tool kit, which offers two ready-to-use workflows for the routine application of genetic methods in conservation management. The workflows were optimized for work load and costs and are accompanied by an easy-to-read and richly illustrated manual with guidelines regarding sampling design, sampling of genetic material, necessary permits, laboratory methods, statistical analyses and documentation of results in a practice-oriented way. The manual also provides a detailed interpretation help for the implementation of the results in conservation management. One workflow deals with the identification of pond-breeding amphibians based on metabarcoding and environmental DNA (eDNA) from water samples. This workflow also discriminates the morphologically similar water frogs (Pelophylaxsp.) and other closely related species (e.g.Triturus cristatusandT. carnifex). The second workflow studies connectivity among populations using microsatellite markers. Its statistical analyses encompass the detection of genetic groups and historical, recent and current dispersal and gene flow. Using the two workflows does not involve academic research institutes; they can be applied by environmental consultancies, laboratories from the private sector, governmental agencies or non-governmental organisations. These and additional conservation genetic workflows will hopefully foster the routine use of genetic methods in conservation management.
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