期刊
CURRENT GENOMICS
卷 17, 期 2, 页码 145-152出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389202917666151116211812
关键词
Cellulases; Chromatin; Promoter; Trichoderma reesei; Rut-C30; Xyr1
资金
- Austrian Science Fund (FWF) [V232-B20, P24851]
- doctoral program of Vienna University of Technology (CatMat)
- Austrian Science Fund (FWF) [V 232, P 24851] Funding Source: researchfish
- Austrian Science Fund (FWF) [V232] Funding Source: Austrian Science Fund (FWF)
The ascomycete Trichoderma reesei is used for the production of plant cell wall-degrading enzymes in industrial scale. The interplay of the transactivator Xyr1 and the repressor Cre1 mainly regulates the expression of these enzymes. During inducing conditions, such as in the presence of sophorose, the transcription of the two major cellulase-encoding genes, cbh1 and cbh2, is activated as well as the expression of xyr1. In the presence of D-glucose carbon catabolite repression mediated by Cre1 takes place and the expression of Xyr1 and the plant cell wall-degrading enzymes is down-regulated. In this study we compare the chromatin status of xyr1, cbh1, and cbh2 promoters in the wild-type strain and the Cre1-deficient strain Rut-C30. Chromatin rearrangement occurs in the xyr1 promoter during induction on sophorose. Chromatin opening and protein-DNA interactions in the xyr1 promoter were detected especially in a region located 0.9 kb upstream the translation start codon, which bears several putative Cre1-binding sites and a CCAAT-box. Moreover, the xyr1 promoter is overall more accessible in a cre1-truncated background, no matter which carbon source is present. This makes the xyr1 regulatory sequence a good target for promoter engineering aiming at the enhancement of cellulase production.
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