期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-020-17859-1
关键词
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资金
- Wellcome Trust [095927/A/11/Z]
- NIH [GM83257, G.M. 131372, GM118486, NS 089662, NS105640, MH115939, F31MH116571, T32GM007223, S10 OD020142]
- Brown-Coxe Postdoctoral Fellowship from Yale School of Medicine
- Wellcome Trust [095927/A/11/Z] Funding Source: Wellcome Trust
Performing multi-color nanoscopy for extended times is challenging due to the rapid photobleaching rate of most fluorophores. Here we describe a new fluorophore (Yale-595) and a bio-orthogonal labeling strategy that enables two-color super-resolution (STED) and 3D confocal imaging of two organelles simultaneously for extended times using high-density environmentally sensitive (HIDE) probes. Because HIDE probes are small, cell-permeant molecules, they can visualize dual organelle dynamics in hard-to-transfect cell lines by super-resolution for over an order of magnitude longer than with tagged proteins. The extended time domain possible using these tools reveals dynamic nanoscale targeting between different organelles.
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