期刊
JOURNAL OF MOLECULAR CELL BIOLOGY
卷 12, 期 11, 页码 894-905出版社
OXFORD UNIV PRESS
DOI: 10.1093/jmcb/mjaa061
关键词
RNF219; CCR4-NOT; protein complex; stem cell differentiation; deadenylation
类别
资金
- National Natural Science Foundation of China [31671343, 31970617, 31970626, 31700718]
- National Key R&D Program of China [2018YFA0800100]
- Natural Science Foundation of Jiangsu Province of China [BK20170020, BK20170663]
- China Postdoctoral Science Foundation [2018M630492]
- Scientific Research Foundation of the Graduate School of Southeast University [YBPY1888]
Regulation of RNA stability plays a crucial role in gene expression control. Deadenylation is the initial rate-limiting step for the majority of RNA decay events. Here, we show that RING finger protein 219 (RNF219) interacts with the CCR4-NOT deadenylase complex. RNF219-CCR4-NOT exhibits deadenylation activity in vitro. RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown, while upregulated after depletion of the CCR4-NOT subunit CNOT10 in mouse embryonic stem (ES) cells. RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation. Our study suggests that RNF219 is a novel interacting partner of CCR4-NOT and required for maintenance of ES cell pluripotency.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据