期刊
MBIO
卷 11, 期 5, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/mBio.02243-20
关键词
COVID-19; SARS-CoV-2; cytotoxic T cells; CD8(+); CD4(+); PD-1; granzyme; perforin; aging
类别
资金
- Stiftung Universitatsmedizin Essen of University Hospital Essen
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces a T cell response that most likely contributes to virus control in COVID-19 patients but may also induce immunopathology. Until now, the cytotoxic T cell response has not been very well characterized in COVID-19 patients. Here, we analyzed the differentiation and cytotoxic profile of T cells in 30 cases of mild COVID-19 during acute infection. SARS-CoV-2 infection induced a cytotoxic response of CD8(+) T cells, but not CD4(+) T cells, characterized by the simultaneous production of granzyme A and B as well as perforin within different effector CD8(+) T cell subsets. PD-1-expressing CD8(+) T cells also produced cytotoxic molecules during acute infection, indicating that they were not functionally exhausted. However, in COVID-19 patients over the age of 80 years, the cytotoxic T cell potential was diminished, especially in effector memory and terminally differentiated effector CD8(+) cells, showing that elderly patients have impaired cellular immunity against SARS-CoV-2. Our data provide valuable information about T cell responses in COVID-19 patients that may also have important implications for vaccine development. IMPORTANCE Cytotoxic T cells are responsible for the elimination of infected cells and are key players in the control of viruses. CD8(+) T cells with an effector phenotype express cytotoxic molecules and are able to perform target cell killing. COVID-19 patients with a mild disease course were analyzed for the differentiation status and cytotoxic profile of CD8(+) T cells. SARS-CoV-2 infection induced a vigorous cytotoxic CD8(+) T cell response. However, this cytotoxic profile of T cells was not detected in COVID-19 patients over the age of 80 years. Thus, the absence of a cytotoxic response in elderly patients might be a possible reason for the more frequent severity of COVID-19 in this age group than in younger patients.
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