期刊
VIRUSES-BASEL
卷 12, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/v12080902
关键词
TBEV; host alternation; neuroinvasiveness; genome mutation; quasispecies; flavivirus adaptation; tick cell line
类别
资金
- Ministry of Education, Youth, and Sports of the Czech Republic INTER-ACTION [LTARF 18021, LTAUSA 18040]
- Czech Science Foundation [GA20-30500S, GA18-27204S]
- Czech research infrastructure for systems biology C4SYS [LM2015055]
- U.K. Biotechnology and Biological Sciences Research Council [BB/P024270/1]
- BBSRC [BBS/E/I/00001741, BB/P024270/1] Funding Source: UKRI
A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) andIxodes ricinustick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.
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