期刊
CURRENT DRUG METABOLISM
卷 17, 期 4, 页码 386-400出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389200217666151210141757
关键词
drug metabolism; HILIC-MS/MS; metabolites; pharmacokinetic study; quantitative bioanalysis; sample preparation
资金
- Qing-Lan Project of Jiangsu Province
- Liu-Da-Ren-Cai-Gao-Feng Project of Jiangsu Province
- Jiangsu Overseas Research & Training Program for University Prominent Young & Middle-aged Teachers and Presidents
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Background: During the development, dosage optimization and safety evaluation of a drug, rapid and precise monitoring of an administered drug and/or its metabolites in biological samples. As drug biotransformation produces more hydrophilic metabolites for the enhancement of drug elimination, it often presents a challenge for traditional reversed-phase liquid chromatography (RPLC) separation. Because hydrophilic interaction liquid chromatography (HILIC) is capable of retaining polar compounds and is readily compatible with mass spectrometry (MS), HILIC has been used as a complementary separation technique to RPLC for the analysis of polar metabolites, especially polar drugs and their metabolites. Methods: We undertook a comprehensive search of databases for HILIC-related research literature. The characteristics including method, result and innovation of screened papers were described, and an inductive and analytical method was applied to analyze those studies using a reasonable framework. Results: This paper covers core aspects of the HILIC-MS/MS method and the overall profile of its application in drug and/or its metabolites analysis by reviewing 102 papers. The emphasis of this paper has been placed on the applications of the HILIC-MS/MS method in quantitative bioanalysis of drugs alone or along with their metabolites in drug metabolism studies in recent years. As a fundamental and critical step of the bioanalytical method, conventional sample preparation techniques of biological matrices for the HILIC-MS/MS analysis of drugs and/or their metabolites are also briefly featured. Conclusion: The findings of this review confirm the powder of HILIC-MS/MS methods in the separation and quantification of drugs and/or their metabolites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据