4.5 Article

Identification and characterization of two bacteriophages with lytic activity against multidrug-resistant Escherichia coli

期刊

VIRUS RESEARCH
卷 291, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.virusres.2020.198196

关键词

Bacteriophage; Multidrug-resistant; Escherichia coli; Cell cytotoxicity; Genome sequencing

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资金

  1. Natural Science Foundation of Hubei Province of China [2020DFE025, 2019AHB068, 2018CFB701]
  2. Open Project of Hubei Key Laboratory of Wudang Local Chinese Medicine Research [WDCM2018009]
  3. Innovative Team Project from the Institute of Medicine and Nursing at Hubei University of Medicine [2017YHKT02]

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This study isolated and characterized two new bacteriophages capable of infecting multidrug-resistant E. coli, demonstrating rapid replication, high stability, and no obvious cytotoxicity. These phages show potential as therapeutic candidates for treating multidrug-resistant E. coli in the future.
Escherichia coli is an opportunistic bacterial pathogen that causes a wide range of nosocomial infections. The emergence of multidrug resistance in E. coli poses a severe threat to global health. Phage therapies are an alternative method to control multidrug-resistant pathogens, which have been attracting increasing attention. Owing to their ability to lyse bacteria specifically and efficiently, bacteriophages are considered novel antimicrobial agents. In this study, we used multidrug-resistant E. coli as an indicator and isolated, characterized, and compared two new phages of the Siphoviridae family referred to as vB_EcoS_XF and vB_EcoS_XY2. These phages were able to infect several pathogenic multidrug-resistant E. coli strains. A short latent period and large burst size ensured their rapidly reproduction in host cells. Their tolerance of high temperatures and high pH levels meant that remained stable when used to control pathogenic E. coli strains. No obvious cytotoxicity was observed when either HEK293 T or A549 cells were incubated with these two phages. Mass spectrometry analysis allowed us to identify several phage-encoded proteins. Genomic analysis revealed that no toxic proteins or antibiotic proteins were encoded. Genome comparison and phylogenetic analysis indicated that the phages identified show high similarity with E. coli phages of the genus Kagunavirus. The desirable characteristics of the novel phages identified make them good potential therapeutic candidates, and components of phage cocktails to treat multidrugresistant E. coli in the future.

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