期刊
CURRENT BIOLOGY
卷 26, 期 2, 页码 195-206出版社
CELL PRESS
DOI: 10.1016/j.cub.2015.11.056
关键词
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资金
- research grants Shriners [8770]
- Cystic Fibrosis Foundation [11P0]
- NIAID [R33AI105902, R56AI063433]
- Shriners Hospitals Research Fellowship [8494]
- Swiss National Science Foundation/Swiss Medical Association (FMH) [PASMP3-123226]
- SICPA Foundation
- [NSF-MCB1052234]
- Swiss National Science Foundation (SNF) [PASMP3-123226] Funding Source: Swiss National Science Foundation (SNF)
Bacterial programmed cell death and quorum sensing are direct examples of prokaryote group behaviors, wherein cells coordinate their actions to function cooperatively like one organism for the benefit of the whole culture. We demonstrate here that 2-n-heptyl--hydroxyquinoline-N-oxide (HQNO), a Pseudomonas aeruginosa quorum-sensing-regulated low-molecular-weight excreted molecule, triggers autolysis by self-perturbing the electron transfer reactions of the cytochrome bc(1) complex. HQNO induces specific self-poisoning by disrupting the flow of electrons through the respiratory chain at the cytochrome bc(1) complex, causing a leak of reducing equivalents to O-2 whereby electrons that would normally be passed to cytochrome c are donated directly to O-2. The subsequent mass production of reactive oxygen species (ROS) reduces membrane potential and disrupts membrane integrity, causing bacterial cell autolysis and DNA release. DNA subsequently promotes biofilm formation and increases antibiotic tolerance to beta-actams, suggesting that HQNO-dependent cell autolysis is advantageous to the bacterial populations. These data identify both a new programmed cell death system and a novel role for HQNO as a critical inducer of biofilm formation and antibiotic tolerance. This newly identified pathway suggests intriguing mechanistic similarities with the initial mitochondrial-mediated steps of eukaryotic apoptosis.
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