期刊
CURRENT BIOLOGY
卷 26, 期 21, 页码 2942-2950出版社
CELL PRESS
DOI: 10.1016/j.cub.2016.08.057
关键词
-
资金
- Human Frontier Science Program [RGP0040/2012]
- European Research Council under the European Union/ERC [617233]
- Agence Nationale pour la Recherche [ANR 13-NANO-0011]
- LABEX Who am I?
- University of Singapore
- Singapore Ministry of Education [MOE2015-T2-1-116]
- Mechanobiology Institute
- NUS Graduate School for Integrative Sciences & Engineering (NGS)
- Agence Nationale de la Recherche (ANR) [ANR-13-NANO-0011] Funding Source: Agence Nationale de la Recherche (ANR)
The control of tissue growth, which is a key to maintain the protective barrier function of the epithelium, depends on the balance between cell division and cell extrusion rates [1, 2]. Cells within confluent epithelial layers undergo cell extrusion, which relies on cell-cell interactions [3] and actomyosin contractility [4, 5]. Although it has been reported that cell extrusion is also dependent on cell density [6, 7], the contribution of tissue mechanics, which is tightly regulated by cell density [8-12], to cell extrusion is still poorly understood. By measuring the multicellular dynamics and traction forces, we show that changes in epithelial packing density lead to the emergence of distinct modes of cell extrusion. In confluent epithelia with low cell density, cell extrusion is mainly driven by the lamellipodia-based crawling mechanism in the neighbor non-dying cells in connection with large-scale collective movements. As cell density increases, cell motion is shown to slow down, and the role of a supracellular actomyosin cable formation and its contraction in the neighboring cells becomes the preponderant mechanism to locally promote cell extrusion. We propose that these two distinct mechanisms complement each other to ensure proper cell extrusion depending on the cellular environment. Our study provides a quantitative and robust framework to explain how cell density can influence tissue mechanics and in turn regulate cell extrusion mechanisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据