期刊
TRANSFUSION
卷 61, 期 1, 页码 12-16出版社
WILEY
DOI: 10.1111/trf.16119
关键词
convalescent donor selection; COVID-19; ELISA correlation; severe acute respiratory syndromecoronavirus 2; virus neutralization
类别
资金
- European Community
- Takeda group of companies
The study analyzed antibodies in 100 convalescent plasma units for COVID-19, finding that high-titer plasma units tended to come from donors with more severe disease, older age, and male sex. However, there were limitations in the correlation between ELISA and neutralization assays, and further cases are needed to confirm the functional significance of this finding.
Background Transfusion of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) convalescent plasma is a promising treatment for severe coronavirus disease 2019 (COVID-19) cases, with success of the intervention based on neutralizing antibody content. Measurement by serologic correlates without biocontainment needs as well as an understanding of donor characteristics that may allow for targeting of more potent donors would greatly facilitate effective collection. Study Design and Methods One hundred convalescent plasma units were characterized for functionally active SARS-CoV-2 neutralizing antibodies, as well as for SARS-CoV-2 binding antibodies, with the intention to establish a correlation between the functionally more relevant neutralization assay and the more accessible enzyme-linked immunosorbent assay (ELISA). Donor demographics such as COVID-19 severity, age, and sex were correlated with antibody titers. Results A mean neutralization titer 50% of 230 (range, <8-1765) was seen for the 100 convalescent plasma units, with highly significant (P < .0001) yet quantitatively limited (R-2= 0.2830) correlation with results of the ELISA. Exclusion of units with particularly high titers (>500) from analysis improved correlation (R-2= 0.5386). A tendency of higher-titer plasma units from donors with increased disease severity, of advanced age, and of male sex was seen, yet the functional relevance of this difference is questionable. Conclusion The ELISA-based correlation to neutralization titer enabled a threshold proposal that could be used to eliminate lower-titer units from the clinical supply for COVID-19 treatment. Disease severity may be associated with the development of higher titers of neutralizing antibodies, although larger case numbers will be needed for additional confirmation.
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