期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 130, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2020.115984
关键词
COVID-19 diagnoses; Polymerase chain reaction; Microfluidics; Miniaturization; Point of care; Future of PCR
资金
- SAFEA, P.R. China [W099109]
- Ministry of Science and Technology, P.R. China [2018YFE010900]
- Grant Agency of the Czech Republic [17-20716S]
- Ministry of Education, Youth and Sports of the Czech Republic [CZ.02.1.01/0.0/0.0/16_013/0001728]
- Research Grant Council of Hong Kong [16209316, 16201017]
- Ministry of Education, Youth and Sport of the Czech Republic [LTACH19005, Q25/LF1]
- Ministry of Health of the Czech Republic [RVO-VFN 64165]
Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据